A case of pediatric ALL with t(16;21)(p11.2;q22) and FUS-ERG rearrangement

a total leukocyte count (TLC) of 2.9×10/L. On day 70, he completed his second phase of induction chemotherapy and was started on G-GSF therapy for severe neutropenia (TLC: 0.76×10/L). On day 73, his peripheral blood smear showed marked leukopenia with a predominance of lymphocytes and 2% blasts without any left shift or increased granulations. This lack of prototypical G-CSF response along with previous MRD positivity and presence of 2% blasts led to a suspicion of relapse, and bone marrow examination was advised. Marrow aspirate showed hypocellular marrow with 14% blasts, and focal areas showed up to 25% blasts (Fig. 1B), along with a lack of a maturing myeloid component, which suggested relapse. Trephine biopsy, in contrast, showed hypocellularity with only a few scattered CD34 positive blasts. This necessitated flow cytometric evaluation, where the blasts showed negativity for B lymphoblastic markers and expressed myeloid markers, including CD13, CD33, and CD34, suggesting that they were regenerating myeloblasts (Fig. 1C-F). No further therapy was administered and the patient was kept on close hematological follow-up. The counts recovered after 4 days, with the typical responses of a left shift and increased granulation. Follow-up marrow examination after complete recovery of blood counts, 10 days after discontinuing G-CSF therapy, revealed cellular marrow elements with 4% blasts (regenerating) and negative MRD (<0.01%). Post-G-CSF blastocytosis is a problematic and confusing issue that is difficult to differentiate from disease recurrence and hemopoietic recovery. In our case, there were regenerating blasts (>20%) in the bone marrow, with an absence of telltale G-CSF-induced changes, such as presence of immature granulocytes, monocytosis, or prominent granulations on peripheral smear. To conclude, the regenerating blast percentage may increase after G-CSF therapy to >20%, without concomitant increases in other mature myeloid precursors. The lag time before complete response to G-CSF therapy may be as long as 10 days, as seen in our case, posing potential problems in management and a challenge in therapeutic decisions. A careful morphological assessment of the blasts at the time of diagnosis, aided by awareness of these G-CSF-associated aberrant patterns and flow cytometric characterization can help guide management decisions.